- 1). Check the laboratory fire extinguisher, safety shower and eyewash station to ensure they are all in working order in case of a spill during the experiment. Don neoprene gloves and safety goggles prior to working with buffer-solution chemicals.
- 2). Prepare the separate components of the buffer solution by adding 150 mg of sodium chloride to a graduated cylinder. Pour 40 mL polyethylene glycol into a second graduated cylinder. Prepare the two remaining cylinders with 55 mg Tris(hydroxymethyl) aminomethane and 150 mg Tris(hydroxymethyl) aminomethane hydrochloride, respectively.
- 3). Pour sodium chloride into a beaker. Add the Tris(hydroxymethyl) aminomethane and Tris(hydroxymethyl) aminomethane hydrochloride and mix the dry ingredients with a glass stirring rod. Slowly add the polyethylene glycol while continuing to stir.
- 4). Add 20 mL distilled water to help the solid chemicals dissolve. Stir with a glass stirring rod until the solution is thoroughly mixed.
- 5). Perform a pH test to check the neutrality of the Cadenza buffer. The solution should indicate a pH level near 7.0.
- 6). Store the Cadenza buffer in a glass container with an airtight lid to prevent evaporation.
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