Preparing a Glucose Solution

• 1). Determine the total volume you will need. Here, we will assume you are making 1,000ml. You will also need to determine the percentage glucose solution you will need. This will depend on the experiment or procedure you are performing.
  
  
• 2). To determine the amount of powdered glucose you will need to make a solution of a given percent, you multiply (mass/volume) * volume. So, in order to make up 1,000ml of a 40-percent glucose solution, you would multiply (40g glucose/100ml) * 1,000ml = 400g glucose. To make a 15-percent solution, you would multiply (15g/100ml) * 1,000ml = 150g glucose. For a five-percent solution, you would multiply (5g/100ml) * 1,000ml = 50g glucose.
  
  
• 3). Put 500ml deionized water into a 1,000ml beaker, along with a stir bar. Place the beaker on a hot plate and turn on the heat and stir functions. You do not want the water to boil, but you will need it to get hot, or else the glucose will not go into solution.
  
  
• 4). Measure out the correct amount of glucose and add this to the beaker. You will need to continue stirring the solution over heat for a few minutes after adding the glucose. Add deionized water to bring the volume up to 1,000ml.